Thank you for your question. Istekhara doesnt have codified rules as it is one of the occult sciences. Rather, it has recommendations for best practice.
When istekhara is performed with the Qur'an or other means it is usually to clarify a situation and so if there are multiple matters that confuse the result of the istekhara then it would be wise to separate the matters and take separate istekharas for each. If there are lots of factors that a person wants to include in the intention and doesn't want to specify each one, but then uses a question that incorporates all of them while leaving the result of the istekhara clear that would be alright.
Sometimes, when we are faced with a difficult decision, there really isn't one choice that is good (although there might be one choice that is less bad than the other).
So, it can be good just to make a decision and stick with it, and have faith that things will work out for the best, and as they are meant to.
If you genuinely feel you made a wrong decision, it is good to talk to someone trustworthy and get their view and see if you should do anything different, but it is better not to keep going over it again and again, as that can feed anxiety. Sometimes part of life is just making a decision and moving on.
If it is possible to change your mind, you can do that, but it is better just to decide once and for all and then set the issue to rest. Going back and forth on a decision can be hurtful to yourself and also to other people involved, such as in family matters, because it keeps them in limbo and from moving on.
Although anxiety is a normal response to difficult situations, it doesn't help yourself or the situation. There are various medical and non-medical ways to try to help with anxiety (which you can read online about). So it is good to try to address that too.
Your feeling intense anxiety and distress about carrying on the result of the Istekhara, means that you were not reluctant about the matter but you were against it. This means that you did not need an Istekhara right from beginning. Istekhara then is invalid, because you had already decided against the matter.
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